Biobest Laboratories Ltd

Stem Cell Process

The image below shows an overview of the stem cell therapy process.

The first step in the process is to obtain a bone marrow sample. Bone marrow is a good source of mesenchymal stem cells which can be used for soft tissue repair. The sample is taken by the vet using standing sedation, there is no need for general anaesthesia. Bone marrow is typically taken from either the sternum or tuber coxae of the injured animal. Biobest have recently been granted approval for the use of adipose tissue as an alternative source of mesenchymal stem cells.

The sample is then sent to Biobest for processing. On arrival at the laboratory our team of cell culturists examine the sample to ensure that there has not been any deterioration in transit. The bone marrow is then centrifuged to separate it into its constituent parts. The part which is richest in stem cells is transferred to a tissue culture flask and over the next few days the stem cells will attach to the flask and start growing. After 7-10 days the cells will usually have reached a stage where they are ready to be split into larger flasks so we can obtain the required number of cells for implantation. Cells will typically be ready to dispatch after 3-4 weeks.

Prior to release a number of QC checks are performed to ensure the cells are sterile, viable and the correct number of cells are present. Cells are then packaged in a validated transport container to minimize degredation during transit and are returned to the practice for delivery the following day. The vet then implants the cells back into the lesion and the horse can begin a rehabilitation programme.

If you have never used stem cell therapies before but would like to try we are currently offering new customers one free stem cell culture by using the voucher at the end of this article. Please note that this is for veterinary practices only and the offer is strictly limited to one treatemnt per new customer.