For information on testing and sample requirements please click here.
Cattle sera can be tested for viral antibodies to screen animals for freedom from infection, to test paired acute and convalescent samples for evidence of recent infection, or on a herd basis to obtain information about disease prevalence. For information on HiHealth Herdcare, our CHeCS registered Cattle Health Scheme click here.
Bovine Viral Diarrhoea / Mucosal Disease
We offer a BVD antibody and a BVD antigen ELISA. Where a persistently infected animal is suspected it is usually most cost effective to screen animals for BVD antibody and then test those with low or negative antibody titres for virus. Suitable samples for test for BVD antibody include serum, plasma, individual or bulk milk samples. For detection of BVD virus we can use either serum, plasma or ear tissue. Antigen positive animals may have a transient acute infection or be persistently infected, and a second sample after approximately 3 weeks should be used before animals are definitely identified as persistently infected. We can also perform the reference tests for antibody and virus (Virus Neutralisation and Virus Isolation respectively) where necessary. As well as BVD Ag ELISA we can also offer a PCR test. Our BVD ELISAs and PCR are ISO:17025 accredited.
We have recently launched a new BVD management tag and test service. Further information can be found here.
Infectious Bovine Rhinotracheitis (IBR)
Antibodies to IBR are detected by an ISO:17025 accredited ELISA. Suitable samples for test include serum, plasma, and individual or bulk milk. We also offer an ISO:17025 accredited IBR gE Blocking Antibody ELISA. This allows differentiation between antibodies to normal and gE deleted virus vaccine strains. Suitable samples for test include serum or plasma. We can also perform the reference tests for antibody of Virus Neutralisation where this is necessary to confirm marginal positive or negative ELISA results.
Antibodies to Mycobacterium paratuberculosis are detected by and ISO:17025 accredited ELISA. Suitable samples for test include serum, plasma, individual or bulk milk samples. We also offer Johne's PCR on faecal samples and bulk milk to confirm the presence of MAP, this test is also ISO:17025 accredited. It may also be used as an additional test for animals entering a Johne's accredited herd.
Antibodies to Leptospira hardjo are detected by an ISO:17025 accredited ELISA. Suitable samples for test include serum, plasma, and individual or bulk milk.
Antibodies to Mycoplasma bovis are detected in serum by ELISA. Suitable test samples include serum or plasma.
Antibodies to RSV are detected in serum by ELISA. Suitable test samples include serum or plasma.
Antibodies to PI3 are detected in serum by ELISA. Suitable test samples include serum or plasma.
Antibodies in milk samples
The ELISAs for IBR, BVD, Johne's and leptospirosis are validated for use with single animal or bulk milk samples. In a single animal they can be used to determine infection status; in bulk milks they can be used to obtain information on herd infection.
IgG antibodies to bovine Neospora can be detected by an ELISA. Suitable samples for test include serum or plasma. This test can assist the diagnosis of abortion due to Neospora.
A Neutralisation test is available to determine the levels of neutralizing antibody to Bovine Rotavirus. Suitable samples for test include colostrum or milk. There is also an ELISA available to detect group A Rotavirus antigen. Faeces must be submitted for this test.
A Neutralisation test is available to determine the levels of neutralizing antibody to Bovine Coronavirus. Suitable samples for test include colostrum or milk. There is also an ELISA to detect Bovine Coronavirus antigen. Faeces must be submitted for this test.
An ELISA is available for the detection of antibodies to Fasciola hepatica (liver fluke). Suitable samples include serum, plasma and milk. We also offer a faecal coproantigen test for liver fluke in sheep and cattle.
An ELISA is available to determine the level of Ostertagia ostertagi antibodies in milk samples.
An AGID test is used to measure the level of total IgG in serum samples.
Ruminant Respiratory PCR Package
This respiratory package covers 8 viral and bacterial pathogens. It can be run on respiratory swabs, BAL and tissue samples. The test is an alternative to IFAT testing for viral antigens and bacterial culture. Please click here for further details and sampling guidelines. The targets detected are:
BoHV-1 (causative agent of IBR)
Biobest offer a 16-plex PCR for the detection of the most common Mastitis pathogens. Further details can be found on our Bactoproof pages.